APFIVE PCR Primer Orientation
During PCR, primers anneal to template DNA strands to initiate replication. If a researcher accidentally designs primers that anneal in the wrong orientation, what would be the consequence?
A
The hydrogen bonds between base pairs would be reversed
B
A-T pairs would form three hydrogen bonds instead of two
C
The antiparallel structure would convert to parallel
D
DNA synthesis would not occur in the desired direction
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