Amino Acid Substitution and Enzyme Function
A single nucleotide substitution in a gene results in the replacement of a hydrophobic amino acid with a hydrophilic amino acid within the active site of the encoded enzyme. Which of the following best explains the most likely consequence of this substitution?
A
No effect as tertiary structure compensates for local changes
B
Enhanced catalytic activity due to increased water accessibility
C
Increased protein stability through new hydrogen bonding
D
Altered substrate binding capacity due to disrupted hydrophobic interactions
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