Vacuolar Response to Osmotic Stress
A plant is genetically engineered to express a fluorescent protein that binds to phosphatidylinositol phosphates in membranes. After the plant is subjected to osmotic stress, increased fluorescence is observed at the tonoplast. This observation most directly supports which of the following conclusions about vacuolar function?
A
Vacuoles increase in size during osmotic stress
B
Membrane lipid composition changes during osmotic signaling
C
Protein synthesis increases at the tonoplast
D
Vacuolar pH becomes more alkaline during stress
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