ATP Synthesis and Proton Gradient
In an experiment using isolated thylakoid membranes, the thylakoid lumen is set to a pH of 4, while the surrounding solution is set to a pH of 8. Although the electron transport chain is inhibited, ATP synthase is fully functional. Which of the following best predicts the outcome if ADP and inorganic phosphate (Pi) are added to the system?
A
Oxygen would be released as water is split
B
No ATP synthesis would occur without electron transport
C
ATP would be synthesized until the proton gradient dissipates
D
NADP+ would be reduced to NADPH
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