APFIVE Enzyme Kinetics and Substrate Concentration
In an experiment, students observe that the rate of an enzyme-catalyzed reaction initially increases as substrate concentration increases, but eventually levels off despite additional substrate. Which explanation BEST accounts for this observation?
A
All available enzyme active sites have become saturated with substrate molecules
B
The reaction has reached chemical equilibrium with equal forward and reverse rates
C
The enzyme has become permanently inactivated due to product inhibition
D
The enzyme has been completely consumed by the reaction
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