Enzyme Kinetics and Substrate Concentration
In an enzyme-catalyzed reaction, the rate increases as substrate concentration rises but eventually plateaus, even with the addition of more substrate. Which of the following best explains this observation?
A
All available enzyme active sites have become saturated with substrate molecules
B
The enzyme has become permanently inactivated due to product inhibition
C
The reaction has reached chemical equilibrium with equal forward and reverse rates
D
The enzyme has been completely consumed by the reaction
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