APFIVE Ligand Receptor Binding Specificity
In an experiment to study cell-to-cell communication, a student applies a fluorescently labeled ligand that should bind to a specific receptor on neighboring cells. However, no fluorescence is observed on the neighboring cells. The ligand and its concentration were confirmed to be correct. What could be the conceptual mistake?
A
The neighboring cells may not express the receptor for the ligand.
B
The fluorescent label degraded before the experiment could be observed.
C
The microscope used was not sensitive enough to detect the fluorescence.
D
The ligand was not applied directly to the cells.
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