Ligand Receptor Binding Specificity
In an experiment on cell signaling, a student treats a culture of cells with a fluorescently labeled ligand intended to bind to a specific receptor. No fluorescence is detected on the cells. If the integrity and concentration of the ligand are correct, which of the following is the most plausible conceptual explanation for this result?
A
The microscope used was not sensitive enough to detect the fluorescence.
B
The neighboring cells may not express the receptor for the ligand.
C
The fluorescent label degraded before the experiment could be observed.
D
The ligand was not applied directly to the cells.
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