APFIVE Interpreting PCR Results
A scientist is using PCR to detect a bacterial pathogen in environmental samples. After 30 cycles, the results show no amplification. Which of the following is the most likely explanation for this negative result?
A
The pathogen DNA is absent or below detection threshold
B
DNA polymerase works too efficiently in the reaction
C
The PCR machine amplified too many cycles
D
The annealing temperature was too specific
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