APFIVE Mechanism of RNA Interference
A student uses RNA interference (RNAi) to target the mRNA of a specific gene, but observes no change in the level of gene expression. Which of the following is the most likely explanation for the ineffectiveness of the RNAi treatment?
A
The RNAi molecules were not complementary to the target mRNA sequence.
B
The RNAi molecules targeted exons instead of introns in the mRNA.
C
RNAi molecules cannot enter the nucleus where gene expression is regulated.
D
The target gene was duplicated, and only one copy was targeted by RNAi.