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AP Biology/Unit 6: Gene Expression and Regulation
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Prokaryotic Polymerase on Eukaryotic DNA
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In an experiment, prokaryotic DNA polymerase is added to a eukaryotic DNA template. Replication is observed to initiate, but the rate of nucleotide incorporation is significantly slower than expected. What is the most likely explanation for this observation?

A

The student forgot to add the RNA primase to the reaction mixture.

B

Prokaryotic DNA polymerase lacks the processivity required for eukaryotic templates.

C

The eukaryotic DNA template was missing an origin of replication.

D

Prokaryotic DNA polymerase cannot add nucleotides to a 3’ end.

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