APFIVE Prokaryotic Polymerase on Eukaryotic DNA
In an experiment, prokaryotic DNA polymerase is added to a eukaryotic DNA template. Replication is observed to initiate, but the rate of nucleotide incorporation is significantly slower than expected. What is the most likely explanation for this observation?
A
The student forgot to add the RNA primase to the reaction mixture.
B
Prokaryotic DNA polymerase cannot add nucleotides to a 3’ end.
C
The eukaryotic DNA template was missing an origin of replication.
D
Prokaryotic DNA polymerase lacks the processivity required for eukaryotic templates.
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