APFIVE Eukaryotic and Prokaryotic Transcription
In an experiment, a student introduces a gene for a eukaryotic transcription factor into bacteria to activate a specific operon. Although the gene is successfully integrated and expressed, transcription of the target operon does not increase. Which of the following best explains this observation?
A
The transcription factor was not expressed at high enough levels in the bacteria.
B
The operon was already being maximally transcribed by the bacteria.
C
Eukaryotic transcription factors are not compatible with bacterial RNA polymerase.
D
The bacterial operon lacks the necessary enhancer sequences for the transcription factor.
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