Enzyme Saturation and Vmax

Increasing nucleotide concentration indefinitely will always result in reaction rates that surpass $V_{max}$ due to substrate excess.

The Michaelis-Menten model indicates that at high substrate concentrations, further increases do not significantly affect the reaction rate.

In practical experiments, nucleotide concentrations are maintained at levels that bring the reaction rate close to, but not exceeding, $V_{max}$.

When the substrate concentration is significantly larger than $K_m$, the reaction rate saturates and approaches $V_{max}$.