Eukaryotic and Prokaryotic Transcription
A student is studying gene regulation in bacteria and uses a eukaryotic transcription factor in an attempt to activate transcription of a bacterial operon. Despite correct insertion of the transcription factor gene into the bacterial genome, the operon’s expression does not increase. What is the most likely reason for this outcome?
A
The bacterial operon lacks the necessary enhancer sequences for the transcription factor.
B
The transcription factor was not expressed at high enough levels in the bacteria.
C
The operon was already being maximally transcribed by the bacteria.
D
Eukaryotic transcription factors are not compatible with bacterial RNA polymerase.
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