Interpreting mRNA Splicing Experiment Results
To study the effect of a mutation on mRNA splicing, a researcher introduces it into a cell line. The researcher expects an increase in intron retention but observes no change in the splicing patterns of the mRNA transcripts. Which potential oversight in the experimental design could account for this result?
A
They overlooked the presence of alternative splicing pathways.
B
They used a cell line that lacks the machinery for alternative splicing.
C
They did not consider that compensatory splicing factors might correct the splicing error.
D
They assumed that the mutation would directly affect splicing machinery.