APFIVE Interpreting PCR Results
A scientist is using PCR to detect a bacterial pathogen in environmental samples. After 30 cycles, the results show no amplification. Which of the following is the most likely explanation for this negative result?
A
The PCR machine amplified too many cycles
B
The annealing temperature was too specific
C
DNA polymerase works too efficiently in the reaction
D
The pathogen DNA is absent or below detection threshold
Question Leaderboard
| Rank | |||||
|---|---|---|---|---|---|
| #1 | hganga30 | 2 | 2 | 0m 46s | 154 |
| #2 | adeoluwa195 | 2 | 3 | 1m 23s | 107 |
| #3 | 541009 | 1 | 1 | 0m 08s | 92 |
| #4 | vivaantiwari49 | 1 | 1 | 0m 09s | 91 |
| #5 | gg33211 | 1 | 1 | 0m 12s | 88 |
| #6 | lilyleonaa | 1 | 1 | 0m 13s | 87 |
| #7 | liyakakar09 | 1 | 1 | 0m 13s | 87 |
| #8 | bakhibakhyari | 1 | 1 | 0m 24s | 76 |
| #9 | erickim9181 | 1 | 1 | 0m 29s | 71 |
| #10 | ik.mar | 1 | 1 | 0m 30s | 70 |
Items per page:
10
1 – 10 of 20