APFIVE Mechanism of RNA Interference
In an experiment to study the regulation of gene expression, a student uses RNAi molecules designed to target the mRNA of a specific gene. However, the gene expression levels remain unchanged. What is the most likely explanation for the failure of RNAi to decrease gene expression?
A
RNAi molecules cannot enter the nucleus where gene expression is regulated.
B
The target gene was duplicated, and only one copy was targeted by RNAi.
C
The RNAi molecules were not complementary to the target mRNA sequence.
D
The RNAi molecules targeted exons instead of introns in the mRNA.
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