APFIVE MicroRNA Gene Regulation Failure
In an experiment to study the role of microRNAs (miRNAs) in gene regulation, a student synthesizes a miRNA that is perfectly complementary to the mRNA of a target gene. However, the expression of the target gene is not affected by the miRNA. What is the most likely reason for the lack of gene regulation by the miRNA?
A
The miRNA was not processed by the Dicer enzyme, preventing its incorporation into RISC.
B
The synthesized miRNA was rapidly degraded by cellular RNases.
C
miRNAs typically regulate gene expression by imperfect base pairing, not perfect complementarity.
D
The target mRNA has a secondary structure that prevents miRNA binding.
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