APFIVE PCR Primer Function and Specificity
During a PCR experiment to amplify a segment of DNA, a student mistakenly uses RNA primers instead of DNA primers. Despite this, the amplification process proceeds. What is the most likely reason that the PCR was still successful?
A
The RNA primers annealed more specifically to the template DNA.
B
DNA polymerase can extend from RNA primers as well as DNA primers.
C
The RNA primers were converted into DNA primers by reverse transcriptase.
D
RNA primers are more stable than DNA primers, leading to efficient amplification.
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