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AP Biology/Unit 6: Gene Expression and Regulation
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PCR Primer Function and Specificity

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In a polymerase chain reaction (PCR) experiment designed to amplify a DNA segment, a student inadvertently uses RNA primers instead of DNA primers. If the amplification proceeds successfully, which of the following best explains this result?

A

The RNA primers annealed more specifically to the template DNA.

B

DNA polymerase can extend from RNA primers as well as DNA primers.

C

RNA primers are more stable than DNA primers, leading to efficient amplification.

D

The RNA primers were converted into DNA primers by reverse transcriptase.

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