APFIVE Prokaryotic Polymerase on Eukaryotic DNA
During an experiment to study DNA replication, a student adds a DNA polymerase from a prokaryotic organism to a eukaryotic DNA template. The student observes that replication initiates, but the rate of nucleotide addition is significantly slower than expected. What is the most likely explanation for this observation?
A
Prokaryotic DNA polymerase lacks the processivity required for eukaryotic templates.
B
The eukaryotic DNA template was missing an origin of replication.
C
Prokaryotic DNA polymerase cannot add nucleotides to a 3’ end.
D
The student forgot to add the RNA primase to the reaction mixture.
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