RNA Interference and Off-Target Effects
In an RNA interference (RNAi) experiment, nematodes were treated with double-stranded RNA (dsRNA) designed to silence a specific target gene. However, instead of the expected decrease, an increase in the target gene’s expression was observed. What is the most likely explanation for this result?
A
The presence of a feedback mechanism that upregulates the gene in response to initial silencing.
B
The dsRNA sequence is identical to a suppressor of the target gene, leading to its unintended inhibition.
C
The dsRNA sequence matches an activator of the gene instead of the gene itself.
D
The dsRNA degrades before it can induce gene silencing.
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